Journal: Journal of Bone and Mineral Research
Article Title: Pain in fibrous dysplasia: identifying nociceptive mechanisms in a preclinical model
doi: 10.1093/jbmr/zjaf039
Figure Lengend Snippet: In vitro cAMP assay (A), NGF ELISA (B), and multiplex assay (C-K). FD cells and control cells were activated with doxycycline (10 μg/mL) or vehicle (phosphate buffered saline) for 24 hr with IBMX. Intracellular cAMP was assessed using cAMP Gs dynamic kit and protein factors were assessed using MSD U-PLEX assay. Two-way ANOVA with Tukey’s multiple comparison test. Graphs are individual values with bars representing median ± IQR. **** p < .0001. Abbreviations: cAMP, cyclic adenosine monophosphate; FD, fibrous dysplasia; IBMX, 3-isobutyl-1-methylxanthine; NGF, nerve growth factor.
Article Snippet: cAMP concentration assay (Cisbio GS Dynamic Kit, PerkinElmer), Meso Scale Discovery Multiplex assay (U-PLEX; MSD), and nerve growth factor (NGF) ELISA (NGF Rapid ELISA kit: mouse; Avantor) were conducted according to manufacturer directions.
Techniques: In Vitro, cAMP Assay, Enzyme-linked Immunosorbent Assay, Multiplex Assay, Control, Saline, Plex Assay, Comparison